Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies.
A simple, rapid, and sensitive reversed-phase high-performance liquid chromatographic (HPLC) method for the measurement of mupirocin concentrations in both skin layers and percutaneous samples has been developed. Mupirocin was extracted from skin layers using PBS–acetonitrile (90:10, v/v). The metho...
| Main Authors: | , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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Elsevier
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/22388 |
| _version_ | 1793400197429067776 |
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| author | Echevarría, L. (Lydia) Blanco-Prieto, M.J. (María José) Campanero, M.A. (Miguel Angel) Santoyo, S. (Susana) |
| author_facet | Echevarría, L. (Lydia) Blanco-Prieto, M.J. (María José) Campanero, M.A. (Miguel Angel) Santoyo, S. (Susana) |
| author_sort | Echevarría, L. (Lydia) |
| collection | DSpace |
| description | A simple, rapid, and sensitive reversed-phase high-performance liquid chromatographic (HPLC) method for the measurement of mupirocin concentrations in both skin layers and percutaneous samples has been developed. Mupirocin was extracted from skin layers using PBS–acetonitrile (90:10, v/v). The method is sufficiently sensitive and repeatable to be used in percutaneous penetration studies. The samples were chromatographed on a View the MathML source mm C8 LiChrospher Select B (5 μm). The mobile phase composition was a mixture of acetonitrile–ammonium acetate 0.05 M (27.5:72.5, v/v) adjusted to pH 6.3 with acetic acid. The analyte was detected at 228 nm and the run time was 11 min. Linearity was confirmed in the concentration range 0.2–20 μg/ml and the limit of detection was 9.5 ng/ml. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-22388 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2012 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-223882020-03-23T17:31:40Z Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. Echevarría, L. (Lydia) Blanco-Prieto, M.J. (María José) Campanero, M.A. (Miguel Angel) Santoyo, S. (Susana) Skin recovery Mupirocin A simple, rapid, and sensitive reversed-phase high-performance liquid chromatographic (HPLC) method for the measurement of mupirocin concentrations in both skin layers and percutaneous samples has been developed. Mupirocin was extracted from skin layers using PBS–acetonitrile (90:10, v/v). The method is sufficiently sensitive and repeatable to be used in percutaneous penetration studies. The samples were chromatographed on a View the MathML source mm C8 LiChrospher Select B (5 μm). The mobile phase composition was a mixture of acetonitrile–ammonium acetate 0.05 M (27.5:72.5, v/v) adjusted to pH 6.3 with acetic acid. The analyte was detected at 228 nm and the run time was 11 min. Linearity was confirmed in the concentration range 0.2–20 μg/ml and the limit of detection was 9.5 ng/ml. 2012-06-01T09:04:31Z 2012-06-01T09:04:31Z 2003-11 info:eu-repo/semantics/article https://hdl.handle.net/10171/22388 eng info:eu-repo/semantics/openAccess application/pdf Elsevier |
| spellingShingle | Skin recovery Mupirocin Echevarría, L. (Lydia) Blanco-Prieto, M.J. (María José) Campanero, M.A. (Miguel Angel) Santoyo, S. (Susana) Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. |
| title | Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. |
| title_full | Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. |
| title_fullStr | Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. |
| title_full_unstemmed | Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. |
| title_short | Development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. |
| title_sort | development and validation of a liquid chromatographic method for in vitro mupirocin quantification in both skin layers and percutaneous penetration studies. |
| topic | Skin recovery Mupirocin |
| url | https://hdl.handle.net/10171/22388 |
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