Specific detection of Brucella DNA by PCR

A PCR assay with primers derived from the 16S rRNA sequence of Brucella abortus was developed. Nine different combinations between six primers were tested. One pair of primers, which amplified a 905-bp fragment, was selected. As little as 80 fg of Brucella DNA was detected by this method. DNAs from...

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Bibliographic Details
Main Authors: Romero, C. (Conchi), Gamazo, C. (Carlos), Pardo, M. (Marisa), Lopez-Goñi, I. (Ignacio)
Format: info:eu-repo/semantics/article
Language:eng
Published: American Society for Microbiology 2013
Subjects:
Online Access:https://hdl.handle.net/10171/29636