Specific detection of Brucella DNA by PCR
A PCR assay with primers derived from the 16S rRNA sequence of Brucella abortus was developed. Nine different combinations between six primers were tested. One pair of primers, which amplified a 905-bp fragment, was selected. As little as 80 fg of Brucella DNA was detected by this method. DNAs from...
Main Authors: | Romero, C. (Conchi), Gamazo, C. (Carlos), Pardo, M. (Marisa), Lopez-Goñi, I. (Ignacio) |
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Format: | info:eu-repo/semantics/article |
Language: | eng |
Published: |
American Society for Microbiology
2013
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Subjects: | |
Online Access: | https://hdl.handle.net/10171/29636 |
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