Structural features governing the activity of lactoferricin-derived peptides that act in synergy with antibiotics against Pseudomonas aeruginosa in vitro and in vivo
Pseudomonas aeruginosa is naturally resistant to many antibiotics, and infections caused by this organism are a serious threat, especially to hospitalized patients. The intrinsic low permeability of P. aeruginosa to antibiotics results from the coordinated action of several mechanisms, such as the p...
| Main Authors: | , , , , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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American Society for Microbiology
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10171/29639 |
| _version_ | 1793400172367052800 |
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| author | Sánchez-Gómez, S. (Susana) Japelj, B. (Bostjan) Jerala, R. (Roman) Moriyon, I. (Ignacio) Fernández-Alonso, M. (Miriam) Leiva, J. (José) Blondelle, S.E. (Sylvie E.) Andrä, J. (Jörg) Brandenburg, K. (Klaus) Lohner, K. (Karl) Martinez-de-Tejada, G. (Guillermo) |
| author_facet | Sánchez-Gómez, S. (Susana) Japelj, B. (Bostjan) Jerala, R. (Roman) Moriyon, I. (Ignacio) Fernández-Alonso, M. (Miriam) Leiva, J. (José) Blondelle, S.E. (Sylvie E.) Andrä, J. (Jörg) Brandenburg, K. (Klaus) Lohner, K. (Karl) Martinez-de-Tejada, G. (Guillermo) |
| author_sort | Sánchez-Gómez, S. (Susana) |
| collection | DSpace |
| description | Pseudomonas aeruginosa is naturally resistant to many antibiotics, and infections caused by this organism are a serious threat, especially to hospitalized patients. The intrinsic low permeability of P. aeruginosa to antibiotics results from the coordinated action of several mechanisms, such as the presence of restrictive porins and the expression of multidrug efflux pump systems. Our goal was to develop antimicrobial peptides with an improved bacterial membrane-permeabilizing ability, so that they enhance the antibacterial activity of antibiotics. We carried out a structure activity relationship analysis to investigate the parameters that govern the permeabilizing activity of short (8- to 12-amino-acid) lactoferricin-derived peptides. We used a new class of constitutional and sequence-dependent descriptors called PEDES (peptide descriptors from sequence) that allowed us to predict (Spearman's ρ = 0.74; P < 0.001) the permeabilizing activity of a new peptide generation. To study if peptide-mediated permeabilization could neutralize antibiotic resistance mechanisms, the most potent peptides were combined with antibiotics, and the antimicrobial activities of the combinations were determined on P. aeruginosa strains whose mechanisms of resistance to those antibiotics had been previously characterized. A subinhibitory concentration of compound P2-15 or P2-27 sensitized P. aeruginosa to most classes of antibiotics tested and counteracted several mechanisms of antibiotic resistance, including loss of the OprD porin and overexpression of several multidrug efflux pump systems. Using a mouse model of lethal infection, we demonstrated that whereas P2-15 and erythromycin were unable to protect mice when administered separately, concomitant administration of the compounds afforded long-lasting protection to one-third of the animals. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29639 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-296392020-12-02T17:12:34Z Structural features governing the activity of lactoferricin-derived peptides that act in synergy with antibiotics against Pseudomonas aeruginosa in vitro and in vivo Sánchez-Gómez, S. (Susana) Japelj, B. (Bostjan) Jerala, R. (Roman) Moriyon, I. (Ignacio) Fernández-Alonso, M. (Miriam) Leiva, J. (José) Blondelle, S.E. (Sylvie E.) Andrä, J. (Jörg) Brandenburg, K. (Klaus) Lohner, K. (Karl) Martinez-de-Tejada, G. (Guillermo) Anti-Bacterial Agents pharmacology Anti-Bacterial Agents therapeutic use Lactoferrin chemistry Peptides pharmacology Peptides therapeutic use Pseudomonas Infections drug therapy Pseudomonas aeruginosa drug effects Female Pseudomonas aeruginosa is naturally resistant to many antibiotics, and infections caused by this organism are a serious threat, especially to hospitalized patients. The intrinsic low permeability of P. aeruginosa to antibiotics results from the coordinated action of several mechanisms, such as the presence of restrictive porins and the expression of multidrug efflux pump systems. Our goal was to develop antimicrobial peptides with an improved bacterial membrane-permeabilizing ability, so that they enhance the antibacterial activity of antibiotics. We carried out a structure activity relationship analysis to investigate the parameters that govern the permeabilizing activity of short (8- to 12-amino-acid) lactoferricin-derived peptides. We used a new class of constitutional and sequence-dependent descriptors called PEDES (peptide descriptors from sequence) that allowed us to predict (Spearman's ρ = 0.74; P < 0.001) the permeabilizing activity of a new peptide generation. To study if peptide-mediated permeabilization could neutralize antibiotic resistance mechanisms, the most potent peptides were combined with antibiotics, and the antimicrobial activities of the combinations were determined on P. aeruginosa strains whose mechanisms of resistance to those antibiotics had been previously characterized. A subinhibitory concentration of compound P2-15 or P2-27 sensitized P. aeruginosa to most classes of antibiotics tested and counteracted several mechanisms of antibiotic resistance, including loss of the OprD porin and overexpression of several multidrug efflux pump systems. Using a mouse model of lethal infection, we demonstrated that whereas P2-15 and erythromycin were unable to protect mice when administered separately, concomitant administration of the compounds afforded long-lasting protection to one-third of the animals. 2013-08-30T11:57:29Z 2013-08-30T11:57:29Z 2011 info:eu-repo/semantics/article https://hdl.handle.net/10171/29639 eng info:eu-repo/semantics/openAccess application/pdf American Society for Microbiology |
| spellingShingle | Anti-Bacterial Agents pharmacology Anti-Bacterial Agents therapeutic use Lactoferrin chemistry Peptides pharmacology Peptides therapeutic use Pseudomonas Infections drug therapy Pseudomonas aeruginosa drug effects Female Sánchez-Gómez, S. (Susana) Japelj, B. (Bostjan) Jerala, R. (Roman) Moriyon, I. (Ignacio) Fernández-Alonso, M. (Miriam) Leiva, J. (José) Blondelle, S.E. (Sylvie E.) Andrä, J. (Jörg) Brandenburg, K. (Klaus) Lohner, K. (Karl) Martinez-de-Tejada, G. (Guillermo) Structural features governing the activity of lactoferricin-derived peptides that act in synergy with antibiotics against Pseudomonas aeruginosa in vitro and in vivo |
| title | Structural features governing the activity of lactoferricin-derived peptides that act in synergy with antibiotics against Pseudomonas aeruginosa in vitro and in vivo |
| title_full | Structural features governing the activity of lactoferricin-derived peptides that act in synergy with antibiotics against Pseudomonas aeruginosa in vitro and in vivo |
| title_fullStr | Structural features governing the activity of lactoferricin-derived peptides that act in synergy with antibiotics against Pseudomonas aeruginosa in vitro and in vivo |
| title_full_unstemmed | Structural features governing the activity of lactoferricin-derived peptides that act in synergy with antibiotics against Pseudomonas aeruginosa in vitro and in vivo |
| title_short | Structural features governing the activity of lactoferricin-derived peptides that act in synergy with antibiotics against Pseudomonas aeruginosa in vitro and in vivo |
| title_sort | structural features governing the activity of lactoferricin-derived peptides that act in synergy with antibiotics against pseudomonas aeruginosa in vitro and in vivo |
| topic | Anti-Bacterial Agents pharmacology Anti-Bacterial Agents therapeutic use Lactoferrin chemistry Peptides pharmacology Peptides therapeutic use Pseudomonas Infections drug therapy Pseudomonas aeruginosa drug effects Female |
| url | https://hdl.handle.net/10171/29639 |
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