In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model
β-carotene is a carotenoid with provitamin A activity and other health benefits, which needs to become bioavailable upon oral intake to exert its biological activity. A better understanding of its behaviour and stability in the gastrointestinal tract and means to increase its bioavailability are hig...
| Main Authors: | , , , , , , , |
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| Format: | ARTÍCULO |
| Language: | es_ES |
| Published: |
2024
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| Online Access: | http://dspace.ucuenca.edu.ec/handle/123456789/44317 https://www.scopus.com/record/display.uri?eid=2-s2.0-85144270817&origin=resultslist&sort=plf-f&src=s&sid=e870743b844449ede83ed77e01e1aacb&sot=b&sdt=b&s=TITLE-ABS-KEY%28In+vitro+bioaccessibility+and+uptake+of%29&sl=15&sessionSearchId=e870743b844449ede83ed77e01e1aacb |
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| author | Ruales Najera, Jeny Cumanda Van de Wiele, Tom Richard Donoso Moscoso, Silvana Patricia Cabezas Teran, Katty Elizabeth Grootaert, Charlotte Ortiz Ulloa, Silvia Johana Van Camp, John Hendrik Van Bockstaele, Filip |
| author_facet | Ruales Najera, Jeny Cumanda Van de Wiele, Tom Richard Donoso Moscoso, Silvana Patricia Cabezas Teran, Katty Elizabeth Grootaert, Charlotte Ortiz Ulloa, Silvia Johana Van Camp, John Hendrik Van Bockstaele, Filip |
| author_sort | Ruales Najera, Jeny Cumanda |
| collection | DSpace |
| description | β-carotene is a carotenoid with provitamin A activity and other health benefits, which needs to become
bioavailable upon oral intake to exert its biological activity. A better understanding of its behaviour and stability
in the gastrointestinal tract and means to increase its bioavailability are highly needed. Using an in vitro
gastrointestinal digestion method coupled to an intestinal cell model, we explored the stability, gastrointestinal
bioaccessibility and cellular uptake of β-carotene from microparticles containing carotenoid extracts derived
from mango by-products. Three types of microparticles were tested: one with the carotenoid extract as such, one
with added inulin and one with added fructooligosaccharides. Overall, β-carotene was relatively stable during
the in vitro digestion, as total recoveries were above 68 %. Prebiotics in the encapsulating material, especially
inulin, enhanced the bioaccessibility of β-carotene almost 2-fold compared to microparticles without prebiotics.
Likewise, β-carotene bioaccessibility increased proportionally with bile salt concentrations during digestion. Yet,
a bile salts level above 10 mM did not contribute markedly to β-carotene bioaccessibility of prebiotic containing
microparticles. Cellular uptake experiments with non-filtered gastrointestinal digests yielded higher absolute
levels of β-carotene taken up in the epithelial cells as compared to uptake assays with filtered digests. However,
the proportional uptake of β-carotene was higher for filtered digests (24 – 31 %) than for non-filtered digests (2 –
8 %). Matrix-dependent carotenoid uptake was only visible in the unfiltered medium, thereby pointing to
possible other cellular transport mechanisms of non-micellarized carotenoids, besides the concentration effect.
Regardless of a filtration step, inulin-amended microparticles consistently resulted in a higher β-carotene uptake
than regular microparticles or FOS-amended microparticles. In conclusion, encapsulation of carotenoid extracts
from mango by-products displayed chemical stability and release of a bioaccessible β-carotene fraction upon
gastrointestinal digestion. This indicates the potential of the microparticles to be incorporated into functional
foods with provitamin A activity. |
| format | ARTÍCULO |
| id | oai:dspace.ucuenca.edu.ec:123456789-44317 |
| institution | Universidad de Cuenca |
| language | es_ES |
| publishDate | 2024 |
| record_format | dspace |
| spelling | oai:dspace.ucuenca.edu.ec:123456789-443172024-03-14T15:34:06Z In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model Ruales Najera, Jeny Cumanda Van de Wiele, Tom Richard Donoso Moscoso, Silvana Patricia Cabezas Teran, Katty Elizabeth Grootaert, Charlotte Ortiz Ulloa, Silvia Johana Van Camp, John Hendrik Van Bockstaele, Filip Microparticles Carotenoids provitamin A Inulin Fructooligosaccharides Caco 2 cells Microencapsulation Filtration β-carotene is a carotenoid with provitamin A activity and other health benefits, which needs to become bioavailable upon oral intake to exert its biological activity. A better understanding of its behaviour and stability in the gastrointestinal tract and means to increase its bioavailability are highly needed. Using an in vitro gastrointestinal digestion method coupled to an intestinal cell model, we explored the stability, gastrointestinal bioaccessibility and cellular uptake of β-carotene from microparticles containing carotenoid extracts derived from mango by-products. Three types of microparticles were tested: one with the carotenoid extract as such, one with added inulin and one with added fructooligosaccharides. Overall, β-carotene was relatively stable during the in vitro digestion, as total recoveries were above 68 %. Prebiotics in the encapsulating material, especially inulin, enhanced the bioaccessibility of β-carotene almost 2-fold compared to microparticles without prebiotics. Likewise, β-carotene bioaccessibility increased proportionally with bile salt concentrations during digestion. Yet, a bile salts level above 10 mM did not contribute markedly to β-carotene bioaccessibility of prebiotic containing microparticles. Cellular uptake experiments with non-filtered gastrointestinal digests yielded higher absolute levels of β-carotene taken up in the epithelial cells as compared to uptake assays with filtered digests. However, the proportional uptake of β-carotene was higher for filtered digests (24 – 31 %) than for non-filtered digests (2 – 8 %). Matrix-dependent carotenoid uptake was only visible in the unfiltered medium, thereby pointing to possible other cellular transport mechanisms of non-micellarized carotenoids, besides the concentration effect. Regardless of a filtration step, inulin-amended microparticles consistently resulted in a higher β-carotene uptake than regular microparticles or FOS-amended microparticles. In conclusion, encapsulation of carotenoid extracts from mango by-products displayed chemical stability and release of a bioaccessible β-carotene fraction upon gastrointestinal digestion. This indicates the potential of the microparticles to be incorporated into functional foods with provitamin A activity. 2024-03-14T15:34:01Z 2024-03-14T15:34:01Z 2023 ARTÍCULO 0963-9969 http://dspace.ucuenca.edu.ec/handle/123456789/44317 https://www.scopus.com/record/display.uri?eid=2-s2.0-85144270817&origin=resultslist&sort=plf-f&src=s&sid=e870743b844449ede83ed77e01e1aacb&sot=b&sdt=b&s=TITLE-ABS-KEY%28In+vitro+bioaccessibility+and+uptake+of%29&sl=15&sessionSearchId=e870743b844449ede83ed77e01e1aacb 10.1016/j.foodres.2022.112301. es_ES application/pdf Food Research International |
| spellingShingle | Microparticles Carotenoids provitamin A Inulin Fructooligosaccharides Caco 2 cells Microencapsulation Filtration Ruales Najera, Jeny Cumanda Van de Wiele, Tom Richard Donoso Moscoso, Silvana Patricia Cabezas Teran, Katty Elizabeth Grootaert, Charlotte Ortiz Ulloa, Silvia Johana Van Camp, John Hendrik Van Bockstaele, Filip In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model |
| title | In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model |
| title_full | In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model |
| title_fullStr | In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model |
| title_full_unstemmed | In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model |
| title_short | In vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/Caco-2 cell model |
| title_sort | in vitro bioaccessibility and uptake of β-carotene from encapsulated carotenoids from mango by-products in a coupled gastrointestinal digestion/caco-2 cell model |
| topic | Microparticles Carotenoids provitamin A Inulin Fructooligosaccharides Caco 2 cells Microencapsulation Filtration |
| url | http://dspace.ucuenca.edu.ec/handle/123456789/44317 https://www.scopus.com/record/display.uri?eid=2-s2.0-85144270817&origin=resultslist&sort=plf-f&src=s&sid=e870743b844449ede83ed77e01e1aacb&sot=b&sdt=b&s=TITLE-ABS-KEY%28In+vitro+bioaccessibility+and+uptake+of%29&sl=15&sessionSearchId=e870743b844449ede83ed77e01e1aacb |
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