Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract
A pilot-scale bioprocess was developed for the production of karlotoxin-enriched extracts of the marine algal dinoflagellate Karlodinium veneficum. A bubble column and a flat-panel photobioreactors (80–281 L) were used for comparative assessment of growth. Flow hydrodynamics and energy dissipation r...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | English |
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Elsevier
2024
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| Online Access: | http://hdl.handle.net/10835/15212 https://doi.org/10.1016/j.biortech.2017.12.101 |
| _version_ | 1789406384249372672 |
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| author | López Rosales, Lorenzo Sánchez Mirón, Asterio García Camacho, Francisco Place, A. R. Chisti, Yusuf Molina Grima, Emilio |
| author_facet | López Rosales, Lorenzo Sánchez Mirón, Asterio García Camacho, Francisco Place, A. R. Chisti, Yusuf Molina Grima, Emilio |
| author_sort | López Rosales, Lorenzo |
| collection | DSpace |
| description | A pilot-scale bioprocess was developed for the production of karlotoxin-enriched extracts of the marine algal dinoflagellate Karlodinium veneficum. A bubble column and a flat-panel photobioreactors (80–281 L) were used for comparative assessment of growth. Flow hydrodynamics and energy dissipation rates (EDR) in the bioreactors were characterized through robust computational fluid dynamic simulations. All cultures were conducted monoseptically outdoors. Bubble column (maximum cell productivity in semicontinuous operation of 58 × 103 cell mL−1 day−1) proved to be a better culture system for this alga. In both reactors, the local EDR near the headspace, and in the sparger zone, were more than one order of magnitude higher than the average value in the whole reactor (=4 × 10−3 W kg−1). Extraction of the culture and further purification resulted in the desired KTXs extracts. Apparently, the alga produced three congeners KTXs: KmTx-10 and its sulfated derivative (sulfo-KmTx-10) and KmTx-12. All congeners possessed hemolytic activity. |
| format | info:eu-repo/semantics/article |
| id | oai:repositorio.ual.es:10835-15212 |
| institution | Universidad de Cuenca |
| language | English |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:repositorio.ual.es:10835-152122024-01-17T09:13:54Z Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract López Rosales, Lorenzo Sánchez Mirón, Asterio García Camacho, Francisco Place, A. R. Chisti, Yusuf Molina Grima, Emilio Dinoflagellates Microalgae Karlodinium veneficum Karlotoxin Computational34 fluid dynamics Photobioreactors A pilot-scale bioprocess was developed for the production of karlotoxin-enriched extracts of the marine algal dinoflagellate Karlodinium veneficum. A bubble column and a flat-panel photobioreactors (80–281 L) were used for comparative assessment of growth. Flow hydrodynamics and energy dissipation rates (EDR) in the bioreactors were characterized through robust computational fluid dynamic simulations. All cultures were conducted monoseptically outdoors. Bubble column (maximum cell productivity in semicontinuous operation of 58 × 103 cell mL−1 day−1) proved to be a better culture system for this alga. In both reactors, the local EDR near the headspace, and in the sparger zone, were more than one order of magnitude higher than the average value in the whole reactor (=4 × 10−3 W kg−1). Extraction of the culture and further purification resulted in the desired KTXs extracts. Apparently, the alga produced three congeners KTXs: KmTx-10 and its sulfated derivative (sulfo-KmTx-10) and KmTx-12. All congeners possessed hemolytic activity. 2024-01-17T09:13:54Z 2024-01-17T09:13:54Z 2017-12-29 info:eu-repo/semantics/article López-Rosales, L., Sánchez-Mirón, A., García-Camacho, F., Place, A. R., Chisti, Y., & Molina-Grima, E. (2018). Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract. Bioresource technology, 253, 94-104. 0960-8524 http://hdl.handle.net/10835/15212 https://doi.org/10.1016/j.biortech.2017.12.101 en https://www.sciencedirect.com/science/article/pii/S0960852417322460 info:eu-repo/semantics/openAccess Elsevier |
| spellingShingle | Dinoflagellates Microalgae Karlodinium veneficum Karlotoxin Computational34 fluid dynamics Photobioreactors López Rosales, Lorenzo Sánchez Mirón, Asterio García Camacho, Francisco Place, A. R. Chisti, Yusuf Molina Grima, Emilio Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract |
| title | Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract |
| title_full | Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract |
| title_fullStr | Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract |
| title_full_unstemmed | Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract |
| title_short | Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract |
| title_sort | pilot-scale outdoor photobioreactor culture of the marine dinoflagellate karlodinium veneficum: production of a karlotoxins-rich extract |
| topic | Dinoflagellates Microalgae Karlodinium veneficum Karlotoxin Computational34 fluid dynamics Photobioreactors |
| url | http://hdl.handle.net/10835/15212 https://doi.org/10.1016/j.biortech.2017.12.101 |
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