The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar
The brucellae are Gram-negative bacteria that cause an important zoonosis. Studies with the main Brucella species have shown that the O-antigens of the Brucella smooth lipopolysaccharide are α-(1 → 2) and α-(1 → 3)-linked N-formyl-perosamine polysaccharides that carry M, A and C (A = M, A>M and A...
| Main Authors: | , , , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | eng |
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Public Library of Science
2013
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| Online Access: | https://hdl.handle.net/10171/29372 |
| _version_ | 1793400008548024320 |
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| author | Zaccheus, M.V. (Mona V.) Ali, T. (Tara) Cloeckaert, A. (Axel) Zygmunt, M. (Michel) Weintraub, A. (Andrej) Iriarte, M. (Maite) Moriyon, I. (Ignacio) Widmalm, G. (Göran) |
| author_facet | Zaccheus, M.V. (Mona V.) Ali, T. (Tara) Cloeckaert, A. (Axel) Zygmunt, M. (Michel) Weintraub, A. (Andrej) Iriarte, M. (Maite) Moriyon, I. (Ignacio) Widmalm, G. (Göran) |
| author_sort | Zaccheus, M.V. (Mona V.) |
| collection | DSpace |
| description | The brucellae are Gram-negative bacteria that cause an important zoonosis. Studies with the main Brucella species have shown that the O-antigens of the Brucella smooth lipopolysaccharide are α-(1 → 2) and α-(1 → 3)-linked N-formyl-perosamine polysaccharides that carry M, A and C (A = M, A>M and A<M) epitopes relevant in serodiagnosis and typing. We report that, in contrast to the B. suis biovar 1 O-antigen used as a reference or to all described Brucella O-antigens, B. suis biovar 2 O-antigen failed to bind monoclonal antibodies of C (A = M), C (M>A) and M specificities. However, the biovar 2 O-antigen bound monoclonal antibodies to the Brucella A epitope, and to the C/Y epitope shared by brucellae and Yersinia enterocolitica O:9, a bacterium that carries an N-formyl-perosamine O-antigen in exclusively α-(1 → 2)-linkages. By (13)C NMR spectroscopy, B. suis biovar 1 but not B. suis biovar 2 or Y. enterocolitica O:9 polysaccharide showed the signal characteristic of α-(1 → 3)-linked N-formyl-perosamine, indicating that biovar 2 may altogether lack this linkage. Taken together, the NMR spectroscopy and monoclonal antibody analyses strongly suggest a role for α-(1 → 3)-linked N-formyl-perosamine in the C (A = M) and C (M>A) epitopes. Moreover, they indicate that B. suis biovar 2 O-antigen lacks some lipopolysaccharide epitopes previously thought to be present in all smooth brucellae, thus representing a new brucella serovar that is M-negative, C-negative. Serologically and structurally this new serovar is more similar to Y. enterocolitica O:9 than to other brucellae. |
| format | info:eu-repo/semantics/article |
| id | oai:dadun.unav.edu:10171-29372 |
| institution | Universidad de Navarra |
| language | eng |
| publishDate | 2013 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | oai:dadun.unav.edu:10171-293722020-03-04T02:24:37Z The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar Zaccheus, M.V. (Mona V.) Ali, T. (Tara) Cloeckaert, A. (Axel) Zygmunt, M. (Michel) Weintraub, A. (Andrej) Iriarte, M. (Maite) Moriyon, I. (Ignacio) Widmalm, G. (Göran) Brucella Gram negative bacteria Lipopolysaccharides Antigens Monoclonal antibodies The brucellae are Gram-negative bacteria that cause an important zoonosis. Studies with the main Brucella species have shown that the O-antigens of the Brucella smooth lipopolysaccharide are α-(1 → 2) and α-(1 → 3)-linked N-formyl-perosamine polysaccharides that carry M, A and C (A = M, A>M and A<M) epitopes relevant in serodiagnosis and typing. We report that, in contrast to the B. suis biovar 1 O-antigen used as a reference or to all described Brucella O-antigens, B. suis biovar 2 O-antigen failed to bind monoclonal antibodies of C (A = M), C (M>A) and M specificities. However, the biovar 2 O-antigen bound monoclonal antibodies to the Brucella A epitope, and to the C/Y epitope shared by brucellae and Yersinia enterocolitica O:9, a bacterium that carries an N-formyl-perosamine O-antigen in exclusively α-(1 → 2)-linkages. By (13)C NMR spectroscopy, B. suis biovar 1 but not B. suis biovar 2 or Y. enterocolitica O:9 polysaccharide showed the signal characteristic of α-(1 → 3)-linked N-formyl-perosamine, indicating that biovar 2 may altogether lack this linkage. Taken together, the NMR spectroscopy and monoclonal antibody analyses strongly suggest a role for α-(1 → 3)-linked N-formyl-perosamine in the C (A = M) and C (M>A) epitopes. Moreover, they indicate that B. suis biovar 2 O-antigen lacks some lipopolysaccharide epitopes previously thought to be present in all smooth brucellae, thus representing a new brucella serovar that is M-negative, C-negative. Serologically and structurally this new serovar is more similar to Y. enterocolitica O:9 than to other brucellae. 2013-06-14T12:13:21Z 2013-06-14T12:13:21Z 2013 info:eu-repo/semantics/article https://hdl.handle.net/10171/29372 eng info:eu-repo/semantics/openAccess application/pdf Public Library of Science |
| spellingShingle | Brucella Gram negative bacteria Lipopolysaccharides Antigens Monoclonal antibodies Zaccheus, M.V. (Mona V.) Ali, T. (Tara) Cloeckaert, A. (Axel) Zygmunt, M. (Michel) Weintraub, A. (Andrej) Iriarte, M. (Maite) Moriyon, I. (Ignacio) Widmalm, G. (Göran) The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar |
| title | The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar |
| title_full | The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar |
| title_fullStr | The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar |
| title_full_unstemmed | The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar |
| title_short | The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar |
| title_sort | epitopic and structural characterization of brucella suis biovar 2 o-polysaccharide demonstrates the existence of a new m-negative c-negative smooth brucella serovar |
| topic | Brucella Gram negative bacteria Lipopolysaccharides Antigens Monoclonal antibodies |
| url | https://hdl.handle.net/10171/29372 |
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